Pre-hypotonic Solution is to be used before the hypotonic during a blood and bone marrow harvest to aid your hypotonic in preventing the tendency of chromosomes to clump or to under spread. The clumping of metaphases during the harvesting procedure often results in the loss of useful metaphases or the loss of entire cultures. Procedures such as FISH which require good well spread metaphases are often hampered by this problem. was developed to address this clumping problem. Optimal metaphase swelling can, therefore, be achieved by your hypotonic solution Makes 1 litre of reagent